How To Look After A Newborn Puppy Without Mother. Take a clean cotton ball and wet it with warm water. This can cause milk to get into his lungs and he will aspirate.
Puppies under the age of about 38 days have a reduced ability to regulate their body. Without the mother available use a warm damp cotton ball or cloth and rub gently over their genitals and anal area. You will need to lower the newborn puppys head to assist with fluid drainage.
Usually the mom dog does this but you will need to do this for a neglected puppy.
Step 4 - Open the puppys mouth by gently inserting your finger between his gums. During the second and third weeks increase the amount of formula in the dropper and spread the feedings to every four to six hours. A normal rectal temperature for a newborn puppy is 95 to 99 degrees Fahrenheit for the first week and 97 to 100 for the second week. Without her and the litter your little one needs a heat source to help maintain a healthy body temperature.